Griinari, J.M. & Bauman, D.E., 1999. Biosynthesis of conjugated linoleic acid its incorporation into meat and milk in ruminants. In: M.P. Yurawecz, M.M. Mossoba, J.K.G. Kramer, M.W Pariza & G.J. Nelson (eds). Advances in conjugated linoleic acid research. Voume 1. AOCS Press, Champaign, IL, 180-200. [ Links ]
Only the named author contributed to the study design, collection of data, analyses, interpretation of results and preparation of the paper.
Bergamo, P., Fedele, E., Iannibeli, L. & Marzillo, G., 2003. Fat soluble vitamin contents and fatty acid composition in organic and conventional Italian dairy products. Food Chem. 82, 625-631. [ Links ]
SAS, 2001. SAS System for Microsoft Windows. Release 8.2. SAS Institute Inc., Cary, NC., USA. [ Links ]
Table 1 gives the composition and nutritional values of feeds. Hemp seeds contain 257 g/kg crude protein (CP) and 316 g/kg fat. The pressing process removes 63% of the fat, increasing the CP to 334 g/kg.
Malondialdehyde is the main product of oxidation of PUFAs. It is widely used as a marker of lipid peroxidation in food and biofluid. The MDA in milk was determined by its reaction with 2-thiobarbituric acid (TBA) (Sigma-Aldrich, St. Louis, USA) at 95°C. MDA and TBA react together to produce a pink chromogen in an acid medium, which was measured photometrically at 532 nm using a spectrophotometer (UV-1201, Shimadzu, Japan). The standard curve to calculate the required concentration of MDA was obtained by acid hydrolysis of 1,1,3,3-tetramethoxypropane (Andrei et al., 2008).
The concentration of LA was greater in the milk of the two groups fed hemp than in C (P <0.05). This is due to the high amount of LA in hemp seed and hempseed cake, in agreement with the findings of Mierlitä (2016), which showed an increased level of this FA in milk of ewes fed hemp seed.
Council Regulation (EC) No 1420/98 of 26 June 1998 amending Regulation (EEC) No 619/71 laying down general rules for granting aid for flax and hemp. [ Links ]
Nelson nods and puts it away. He turns 86 this spring and has a history of emphysema, so Annie, who’s been with Willie for 33 years, tries to get him to look out for his lungs, especially on show days. This can be a problem. “He’s super-generous,” she says, “and if there’s somebody around, he’ll want to offer it and do it with them to make them feel comfortable.”
Sixty-five years after he smoked his first joint, Willie Nelson is America’s most legendary stoner and a walking testament to the power of weed. It may have even saved his life
Sitting with Nelson, you get used to long silences. “Oh, pickin’ a little,” he says when asked about what he’s been up to. He also just finished an album, Ride Me Back Home. The first song is about the 60 horses on his property, which Nelson bought at auction and saved from slaughterhouses. Nelson had showed me some of the horses when I visited five years ago. “Billy Boy is still here,” he says. “We lost Roll Em Up Jack. Wilhelmena the mule is gone. Uh, rattlesnake got her. Babe, you got any of that CBD coffee?”
Nelson is talking in his bunkhouse, a one-floor wood-paneled space across the driveway from the house. Donald Trump is on MSNBC. Nelson picks up his remote, but it’s old and janky, and the button doesn’t really work. “It’s hard to turn him off,” Nelson says.